Durum wheat landraces from Syria

Syrian durum wheat landraces from diverse collection sites were evaluated for agronomic performance under arid conditions over two seasons at four locations, at two levels of nutrient availability.Grain yield differed considerably among locations. Within locations, significant population and fertilizer effects were demonstrated. In most cases, maximum yield was achieved by landraces, which demonstrates the breeding value of local germplasm. In a particular environment, the population effect was nonsignificant for total dry matter production, but significant for straw and grain yield. Nitrogen application was ineffective if moisture availability was the dominant growth limiting factor.Correlations between plant characteristics at evaluation and collection site characteristics were limited, and therefore, their utilization in germplasm evaluation appear limited. The negative relation between grain yield and soil nitrogen content at the collection site may be caused by the comparative disadvantage that germplasm from regions with favourable growing conditions has under low-yielding conditions.Particular environmental conditions at the collection site were no indicator for the response to changed growing conditions. Relatively high grain yields under good and adverse growing conditions excluded each other in all but two cases. Also, it was difficult to relate grain yield to regions of collection, as the response of individual landraces from a certain region to changing growing conditions differed strongly. Differentiation on the basis of landrace groups appears more promising. The Hauran landrace group provided the largest number of populations with relatively high grain yields over diverse environments.     

Differential Adaptation of Complete and Substituted Triticale

Adaptation of seven complete and twelve substituted triticales to specific soil types has been studied, based on a series of twenty trials carried out in 1989 and 1990 across Spain. The nature of the GE interaction for grain yield was revealed by means of the additive main effects multiplicative interaction (AMMI) model and using the soil pH at the different sites as linear covariate. The percentage of the variability explained by the first principal component axis of the AMMI model was 72 and 65 % for the two years, suggesting a specific pattern of adaptation. Soil pH was the single most important environmental factor to explain the adaptation of complete and substituted types. Complete triticales outyielded substituted genotypes in the majority of sites. Triticale adaptation to acid and alkaline soils seems to be largely controlled by the single wheat/rye chromosome 2D(2R) substitution, for which both types differ. Complete triticales seem better adapted to the acid soils, whereas substituted types are, in general, more suited to alkaline soils.     

Epistasis in Maize (Zea mays L.) III. Comparison of Single and Three-Way Crosses for Forage Traits

The importance of epistasis for forage performance and quality traits was studied in early maturing European maize (Zea mays L.) breeding materials. Sixty-six three-way crosses (3W), 33 of the flint x (dent · dent) and 33 of the dem × (flint · flint) type, were compared with the mean of their respective non-parental single crosses () using data obtained in six environments. For each of the 11 traits examined, at least 6 out of the 66 three-way crosses displayed significant (P < 0.05) epistatic deviations, calculated as – 3W. Averaged over crosses and environments, epistatic deviations were highly significant (P < 0,31] for 8 trails, but of minor importance except for grain yield. In general, the variance of epistatie deviations was appreciably smaller than the variance of genotype × environment interactions. Highly significant correlations occurred among epistatie deviations for silking date, dry matter content of ear, and dry matter yield of grain and stover. The reliability of predicting the performance of three-way crosses from 2W as opposed to assessing them directly was investigated as a function of the number of test environments. Disturbing e fleets of epistasis on prediction only became important under intense testing (> 3 or 4 environments), With fewer test environments, both procedures were almost equivalent, due to a smaller influence of genotype × environment interaction on the values, Epistasis is concluded to be of minor importance with regard to the optimum type of hybrid for forage use.     

Relationship between barley grain yield measured in low- and high-yielding environments

Summary The paper addresses the general question of identifying the optimum environment for selection in plant breeding programs for low input agricultural systems. After defining low-yielding and high-yielding environments based on the average grain yield of large numbers of barley genotypes in different cropping seasons, we examined: 1) the phenotypic relationships between the highest yielding genotypes in low- and high-yielding environments, and 2) the genetic correlation coefficients between grain yield in low- and high-yielding environments. The results indicate that the alleles controlling high grain yield in low-yielding conditions are at least partially different from those controlling high grain yield in high-yielding conditions. Therefore, selection in high-yielding environments is expected to produce a negative response or no response in low-yielding environments. This may explain why crop varieties bred under high-yielding conditions failed to have an impact in low-yielding agricultural systems. The results may be extrapolated to systems where environmental concern suggests a reduction of inputs by raising the question of whether crop breeding programs based on selection under high inputs are likely to generate the right type of germplasm for an environmentally friendly agriculture.     

Location-Specific and Across-Location Selections for Seed Yield in Populations of Common Bean, Phaseolus vulgaris L.

Two populations of common bean, Phaseolus vulgaris L., were used to make three independent location-specific selections at Palmira, Quilichao, and Popayán, Colombia. Also, three across-location selections were made by alternating generations between Palmira and Popayan, between Quilichao and Popayán, and among Palmira, Quilichao, and Popayán. The seven highest yielding lines selected from each environment and population along with 14 parents and two checks were evaluated for seed yield, seed weight, maturity, and reaction to an-thracnose, angular leaf spot, and common bacterial blight. Mean squares due to evaluation and selection environments, selected lines, and their interactions were significant for all traits. Mean yields of selected lines were higher than the mean of parents in both populations. Among location-specific selections, lines yielded higher at their respective selection site. Moreover, selections made at Popayán were higher yielding than those made at Quilichao or Palmira when tested across locations and years. Use of Popayán, a relatively wetter and cooler site, either alone or in combination with other locations resulted in higher yield, higher seed weight, and higher resistance to anthracnose. On the average, location-specific selections were lower yielding than the across-location selections. Among across-location selections, those selected between Palmira and Popayán were the highest yielding in both populations. Selections at Palmira and across all three locations resulted in greater stability.     

甘肃地区牛源金黄色葡萄球菌分子鉴定及RAPD分型

本研究目的是分离鉴定引起甘肃地区奶牛乳房炎的金黄色葡萄球菌,掌握其基因型情况。利用16S、23SrRNA保守序列PCR扩增对乳房炎奶样中的金黄色葡萄球菌进行鉴定,并进行RAPD基因分型。结果表明,310份奶样中共分离出金黄色葡萄球菌100株,RAPD结果显示这100株金黄色葡萄球菌均可得到清晰的RAPD指纹图谱,扩增产物在2～7条带之间,具有多种带型组成。通过聚类分析100株菌产生11个基因型,其中Ⅰ型4株,Ⅱ型4株,Ⅲ型10株,Ⅳ型13株,Ⅴ型7株,Ⅵ型24株,Ⅶ型16株,Ⅷ型6株,Ⅸ型4株,Ⅹ型10株,Ⅺ型2株。Ⅵ型为该地区的流行优势菌群,不同牛场各基因型菌株分布有明显差异。本研究说明牛场的环境与养殖条件对病原菌流行传播有明显的影响,这一结果对地区性奶牛乳房炎的防治提供了可靠的理论依据。     

马尾松种源基因型×环境互作及其稳定性的研究

福建省4个地域91个种源10年生时试验结果表明:(1)各点马尾松种源生长上差异十分显著,树高、胸径和材积三个性状的遗传方差份量约占总变异的38.92%—42.75%;(2)种源×地点的效应,树高不明显,材积和胸径达显著水准,而种源基因型效应比较明显,其方差份量约为GE互作份量的1倍;(3)根据Francls和Kanuenbery模型计算种源GE互作效应及其稳定性,将参试种源划分为4种类型,其中高产稳定型种源26个,平均材积遗传增益达42.84%,可在全省各地区推广;(4)广西岑溪和广东罗定两个种源生长最为突出,平均村积遗传增益达88%。     

Molecular characterization of Korean rabies virus isolates

The nucleoprotein (N) and glycoprotein (G) of 11 Korean rabies virus (RABV) isolates collected from animals diagnosed with rabies between 2008 and 2009 were subjected to molecular and phylogenetic analyses. Six isolates originated from domestic animals (cattle and dogs) and five were obtained from wild free-ranging raccoon dogs. The similarities in the nucleotide sequences of the N gene among all Korean isolates ranged from 98.1 to 99.8%, while those of the G gene ranged from 97.9 to 99.3%. Based on the nucleotide analysis of the N and G genes, the Korean RABV isolates were confirmed as genotype I of Lyssavirus and classified into four distinct subgroups with high similarity. Phylogenetic analysis showed that the Korean isolates were most closely related to the non-Korean NeiMeng1025B and 857r strains, which were isolated from rabid raccoon dogs in Eastern China and Russia, respectively. These findings suggest that the Korean RABV isolates originated from a rabid raccoon dog in Northeastern Asia. Genetic analysis of the Korean RABV isolates revealed no substitutions at several antigenic sites, indicating that the isolates circulating in Korea may be pathogenic in several hosts.     

基因型对红三叶异黄酮含量的影响

采用高效液相色谱法,对4个不同红三叶品种的异黄酮含量进行了分析,发现基因型对红三叶的异黄酮总含量影响显著,4个供试材料的异黄酮总含量为:新品系R-1>瑞德>岷山>多丽.基因型对红三叶各异黄酮单体含量变化影响不同,对大豆黄素含量的变化影响最大,对刺芒柄花素含量的变化影响最小.红三叶积累刺芒柄花素的能力最高,其次是鹰嘴豆芽...     

猪戊型肝炎病毒荧光定量PCR检测方法的建立及其分子流行病学

对已发表的戊型肝炎病毒(HEV)不同基因型毒株全序列进行分析,针对ORF3的保守区设计引物和探针优化建立了HEV荧光RT-PCR检测体系。分析表明该反应体系具有良好的扩增效率、特异性和稳定性,且检测灵敏度比普通RT-PCR方法高10～100倍。利用所建立的荧光定量PCR方法对采集自浙江及其周边上海、江苏等地区规模化猪场的样品以及HEV抗体阳性人血清样品进行了HEV核酸检测。其中,77份人血清样本仅有2份为HEV核酸阳性(2.6%)。而猪粪便样品阳性率则高达20.5%(56/273),且所调查的猪场均存在HEV感染。进一步的分子流行特征分析表明,浙江及周边地区的猪HEV毒株多为基因Ⅳ型,与其他地区的Ⅳ型HEV毒株同源性较高(83.4%～89.5%),但它们之间也存在较多的变异位点。猪源HEV毒株PJ-1则与多数Ⅲ型HEV毒株同源性较高(88%),进化分析也表明其为基因Ⅲ型。人血清样品中检测到的2份HEV毒株Human-1与Human-2与本地区Ⅳ型猪源毒株在分子进化关系上具有很高的亲源性,它们分布于同一分支内,而不构成独立分支。以上分析结果表明浙江及其周边地区猪HEV感染较为广泛,且以基因Ⅳ型毒株为主,但也存在...